Flow Cytometry
Multiparameteric Characterization of single cells, particles and microvescicles
What do we do?
The Flow Cytometry core facility offers advanced cellular analysis capabilities, utilizing the Beckman Coulter CytoFLEX S flow cytometer. The CytoFLEX S is equipped with four active lasers (405 nm, 488 nm, 561 nm, and 638 nm) and 13 channels for fluorescence detection, allowing for highly detailed, multi-parameter analysis of a variety of cellular characteristics. With its ability to detect multiple fluorescent markers simultaneously, the CytoFLEX S supports a wide range of applications, from basic to clinical research.
The CytoFLEX S is particularly effective for studying cell cycle progression, apoptosis, and lymphocyte immunophenotyping. Researchers can use the instrument to analyze cell cycle stages (G1, S, G2/M), providing insights into cellular proliferation and responses to various treatments. It is also valuable for apoptosis research, detecting early apoptotic markers such as annexin V binding and caspase activation.
One of the key features of the CytoFLEX S is its sensitivity, particularly with the violet laser, which is ideal for detecting small particles like microvesicles. This is especially useful for studying extracellular vesicles which play an important role in intercellular communication, cancer progression, and immune modulation.
The facility also plans to introduce a cell sorter soon, enabling the isolation of specific cell populations for further analysis, functional assays, and therapeutic applications.
In addition to its technical capabilities, the Flow Cytometry core facility provides expert support and training to ensure users can optimize the flow cytometer for their specific research needs.