Single cell -omics

Services provided

We provide access to the following platforms:

XENIUM - The Xenium In Situ Gene Expression workflows measure gene expression with high sensitivity and subcellular resolution in fresh frozen and FFPE tissue sections. Using pre-designed or custom probe panels, the platform targets hundreds to thousands of genes across a variety of tissue types. This platform uses imaging to detect and decode optical signatures at single cell resolution, eliminating the need for sequencing.

VISIUM - Visium Spatial Gene Expression for formalin fixed & paraffin embedded (FFPE) is a probe-based assay allowing simultaneous analysis of molecular and imaging data from FFPE and Frozen tissue sections. The Visium CytAssist Spatial Gene assay is designed to analyze mRNA in tissue sections sourced from FFPE. It utilizes probes targeting the whole transcriptome.

Each Visium CytAssist Spatial Gene Expression Slide contains Capture Areas with barcoded spots that include oligonucleotides required to capture gene expression probes. The transcriptome probes are hybridized to the tissue. Tissue slides and Visium CytAssist Spatial Gene Expression Slides are loaded into the Visium CytAssist instrument, where the gene expression-ligated probes are released from the tissue onto the CytAssist slides enabling capture by the spatially barcoded oligonucleotides present on the Visium slide surface. Gene expression libraries are generated from each tissue section and sequenced. Spatial Barcodes are used to associate the reads back to the tissue section images for spatial mapping of gene expression.

CHROMIUM - Single cell RNA sequencing (scRNA-seq) measures whole transcriptome gene expression in individual cells, offering a detailed view of how cells function and interact within complex tissues.

• Single-cell 3’RNAsequencing or Single-cell gene Expression Flex (10X Genomics)
• Single-cell Immune profiling-VDJ (10X Genomics)
• Single-cell multiome ATAC + Gene expression (10X Genomics)
• Single-cell ATAC sequencing (10X Genomics)

TECHNIQUES AVAILABLE

Xenium (10X Genomics) for spatial transcriptomics https://www.10xgenomics.com/platforms/xenium

CytAssist for Visium (10X Genomics) for single cell whole transcriptome analysis at spatial resolution https://www.10xgenomics.com/platforms/visium

Chromium (10X Genomics) for single cell -omics https://www.10xgenomics.com/platforms/chromium