Livecyte (Phasefocus)
Imaging platform for real-time live-cell analysis based on quantitative phase ptychography (QPI). It delivers quantitative data on cell proliferation, motility and morphology over time.
The Phasefocus Livecyte provides automatic single-cell segmentation over time in a multiwell assay format. The analysis software delivers quantitative data on parameters related to cell proliferation (cell count, confluence and total mass), cell motility (velocity, directionality and displacement), and morphology (thickness, cell area, perimeter, and sphericity). The instrument is ideal for phenotypic and toxicological screenings. It also
- supports scratch wound healing assays;
- quantifies the effect of different treatments on cell mitotic behaviour, from number of mitosis event, to mitotic time;
- analyzes and quantifies angiogenesis network formation;
- can explore population heterogeneity;
- enables fluorescence analysis, coupling fluorescence signals from labeled cells with ptychographic data. The instrument is configured with filters for fluorescence acquisition in the green range (Ex 470–500 nm; Em 510–540 nm) and red range (Ex 530–580 nm; Em 600–650 nm).