Endocrinology research group – dott.ssa Fanelli

Mass spectrometry division

Research fields

Development and validation of methods for the quantification of small molecules, such as hormones, lipids and metabolites, by liquid chromatography – tandem mass spectrometry. Application of methods to the analysis of human (mainly serum, plasma, dried blood spot and saliva), animal and in vitro samples from studies related to analytical comparison, reference interval generation, characterization of endocrine and non endocrine diseases.

Projects

HarmoSter: interlaboratory comparison study among lc-ms/ms assays for steroid hormone measurements
PRIN2020 “Highlights on the fate of endogenous and exogenous glucocorticoids by liquid chromatography/mass spectrometry. Untangling the metabolic pathways to predict cardiovascular and immune outcomes”.
PRIN2017 “The impact of glucocorticoid Circadian rHytm disRuption on cardiOvascular risk and immuNe dysfunction: pathOphysiology and novel bIoMArkers of Glucocorticoid Exposure in hypercortisolim and hypocortisolism - CHRONO-IMAGE”.
SPIOMET4HEALTH “PCOS in adolescent girls and young women: toward a treatment guided by pathophysiology”.
Analysis of Leydig cell steroidogenesis in function of gonadotropin stimulation and phosphodiesterasis inhibitors.

Publications

Leggi dettagli

1. Fanelli F, Bruce S, Cantù M, Temchenko A, Mezzullo M, Lindner JM, Peitzsch M, Binz PA, Ackermans MT, Heijboer AC, Van den Ouweland J, Koeppl D, Nardi E, Rauh M, Vogeser M, Eisenhofer G, Pagotto U. Report from the HarmoSter study: inter-laboratory comparison of LC-MS/MS measurements of corticosterone, 11-deoxycortisol and cortisone. Clin Chem Lab Med. 2022 Oct 27;61(1):67-77. doi: 10.1515/cclm-2022-0242. Epub ahead of print (IF: 8.49 in 2021).
2. Fanelli F, Cantù M, Temchenko A, Mezzullo M, Lindner JM, Peitzsch M, Hawley JM, Bruce S, Binz PA, Ackermans MT, Heijboer AC, Van den Ouweland J, Koeppl D, Nardi E, MacKenzie F, Rauh M, Eisenhofer G, Keevil BG, Vogeser M, Pagotto U. Report from the HarmoSter study: impact of calibration on comparability of LC-MS/MS measurement of circulating cortisol, 17OH-progesterone and aldosterone. Clin Chem Lab Med, 2022 (DOI: 10.1515/cclm-2021-1028) Epub ahead of print. PMID: 35172417 (IF: 8.49 in 2021).
3. Mezzullo M, Gambineri A, Di Dalmazi G, Fazzini A, Magagnoli M, Baccini M, Vicennati V, Pelusi C, Pagotto U, Fanelli F. Steroid reference intervals in women: influence of menopause, age and metabolism. Eur J Endocrinol 2021; 184:395-407 (DOI: 10.1530/EJE-20-1147) (IF: 6.664 in 2020).
4. Mularoni V, Esposito V, Di Persio S, Vicini E, Spadetta G, Berloco P, Fanelli F, Mezzullo M, Pagotto U, Pelusi C, Nielsen JE, Rajpert-De Meyts E, Jorgensen N, Jorgensen A, Boitani C. Age-related changes in human Leydig cell status. Human Reproduction 2020; 35:2663-2676 (DOI:10.1093/humrep/deaa271) (IF: 6.914).
5. Mezzullo M, Pelusi C, Fazzini A, Repaci A, Di Dalmazi G, Gambineri A, Pagotto U, Fanelli F. Female and male serum reference intervals for challenging sex and precursor steroids by liquid chromatography tandem mass spectrometry. J Steroid Biochem Mol Biol. 2020; 197:105538 (DOI: 10.1016/j.jsbmb.2019.105538)(IF: 4.292).
6. Oriolo C*, Fanelli F*, Castelli S, Mezzullo M, Altieri P, Corzani F, Pelusi C, Repaci A, Di Dalmazi G, Vicennati V, Baldazzi L, Menabò S, Dormi A, Nardi E, Brillanti G, Pasquali R, Pagotto U, Gambineri A. Steroid biomarkers for identifying non-classic adrenal hyperplasia due to 21-hydroxylase deficiency in a population of PCOS with suspicious levels of 17OH-progesterone. J Endocrinol Invest. 2020; 43:1499-1509 (DOI: 10.1007/s40618-020-01235-3)(IF: 4.256).
7. Pelusi C, Fanelli F, Baccini M, Triggiani V, Bartolomeo N, Carbone MD, De Pergola G, Di Dalmazi G, Pagotto U, Pasquali R, Giagulli VA. Effect of clomiphene citrate treatment on the Sertoli cells of dysmetabolic obese men with low testosterone levels. Clin Endocrinol (Oxf). 2019; 92:38-45 (DOI: 10.1111/cen.14122)(IF: 3.380).
8. Di Dalmazi G, Fanelli F, Zavatta G, Ricci Bitti S, Mezzullo M, Repaci A, Pelusi C, Gambineri A, Altieri P, Mosconi C, Balacchi C, Golfieri R, Cosentino ER, Borghi C, Vicennati V, Pasquali R, Pagotto U. The Steroid Profile of Adrenal Incidentalomas: Subtyping Subjects With High Cardiovascular Risk. J Clin Endocrinol Metab. 2019; 104:5519-5528. (doi: 10.1210/jc.2019-00365)(IF: 5.399).
9. Fanelli F, Mezzullo M, Repaci A, Belluomo I, Ibarra Gasparini D, Di Dalmazi G, Mastroroberto M, Vicennati V, Gambineri A, Morselli-Labate AM, Pasquali R, Pagotto U. Profiling plasma N-Acylethanolamine levels and their ratios as a biomarker of obesity and dysmetabolism. Molecular Metabolism. 2018; 14:82-94 (DOI: 10.1016/j.molmet.2018.06.002) (IF: 6.448).
10. Mezzullo M, Fazzini A, Gambineri A, Di Dalmazi G, Mazza R, Pelusi C, Vicennati V, Pasquali R, Pagotto U, Fanelli F. Parallel diurnal fluctuation of testosterone, androstenedione, dehydroepiandrosterone and 17OHprogesterone as assessed in serum and saliva: validation of a novel liquid chromatography-tandem mass spectrometry method for salivary steroid profiling. Clinical Chemistry and Laboratory Medicine 55:1315-1323; 2017 (DOI: 10.1515/cclm-2016-0805) (IF: 3.43).
11. Mezzullo M, Fanelli F, Fazzini A, Gambineri A, Vicennati V, Di Dalmazi G, Pelusi C, Mazza R, Pagotto U, Pasquali R. Validation of an LC-MS/MS salivary assay for glucocorticoid status assessment: Evaluation of the diurnal fluctuation of cortisol and cortisone and of their association within and between serum and saliva. Journal of Steroid Biochemistry and Molecular Biology 163:103-112; 2016 (DOI: 10.1016/j.jsbmb.2016.04.012) (IF: 4.56).
12. Pasquali R, Zanotti L, Fanelli F, Mezzullo M, Fazzini A, Morselli Labate AM, Repaci A, Ribichini D, Gambineri A. Defining Hyperandrogenism in Women With Polycystic Ovary Syndrome: A Challenging Perspective. Journal of Clinical Endocrinology and Metabolism. 101:2013-2022; 2016 (DOI: 10.1210/jc.2015-4009) (IF: 5.46).
13. Büttler RM, Martens F, Fanelli F, Pham HT, Kushnir MM, Janssen MJ, Owen L, Taylor AE, Soeborg T, Blankenstein MA, Heijboer AC. Comparison of 7 Published LC-MS/MS Methods for the Simultaneous Measurement of Testosterone, Androstenedione, and Dehydroepiandrosterone in Serum. Clinical Chemistry 61:1475-1483; 2015 (DOI: 10.1373/clinchem.2015.242859) (IF: 7.46).
14. Fanelli F, Di Lallo VD, Belluomo I, De Iasio R, Baccini M, Casadio E, Ibarra Gasparini D, Colavita M, Gambineri A, Grossi G, Vicennati V, Pasquali R, Pagotto U. Estimation of reference intervals of five endocannabinoids and endocannabinoid related compounds in human plasma by two dimension-LC/MS/MS. Journal of Lipid Research 2012; 53:481-493 (DOI: 10.1194/jlr.M021378) (IF: 4.94).
15. Fanelli F, Belluomo I, Di Lallo VD, Cuomo G, De Iasio R, Baccini M, Casadio E, Casetta B, Vicennati V, Gambineri A, Grossi G, Pasquali R, Pagotto U. Serum steroid profiling by isotopic dilution - liquid chromatography - mass spectrometry: comparison with current immunoassays and reference intervals in healthy adults. Steroids 76:244-253; 2011 (DOI: 10.1016/j.steroids.2010.11.005) (IF: 2.83).

Endocrinology research group – dott.ssa Fanelli

Research fields

Projects

Publications

Team Manager

Flaminia Fanelli

Team

Valentina Bissi

Greta Galante

Ahmed Gaafar