It is designed for precise detection and analysis in microplate-based assays. It supports multiple applications, including fluorescence, absorbance, luminescence, and more.
Spark® Multimode Microplate Reader is an advanced instrument for multimode detection in microplates. This instrument integrates absorbance, fluorescence, and luminescence detection technologies, thereby providing a comprehensive platform for various assay requirements.
Applications:
Molecular Biology Research: UV-Vis absorbance for DNA, RNA, and protein quantification.
Drug Screening & Discovery: Fluorescence intensity (FI), luminescence (LUM), and AlphaScreen/AlphaLISA for high-throughput screening (HTS).
Cell Viability & Cytotoxicity Studies: ATP-based luminescence, resazurin reduction, and MTT/XTT absorbance assays.
Microbial Growth Monitoring: OD 600 absorbance measurements for bacterial and yeast culture analysis.
Protein-Protein & Ligand Binding Studies: Fluorescence polarization (FP), Fluorescence Resonance Energy Transfer (FRET), and time-resolved fluorescence (TRF).
Enzyme Kinetics & Biochemical Assays: Spectrophotometric kinetic assays, TRF, and chemiluminescent enzyme activity assays.
Live Cell-Based Assays: Luminescence and fluorescence-based kinetic cell tracking, CO₂/O₂-controlled assays for metabolic studies.
Reporter Gene Assays: Firefly and Renilla luciferase luminescence assays.
Immunoassays (e.g., ELISA): Absorbance and fluorescence detection for antigen-antibody interactions.
Tecan Spark also offers temperature control, gas regulation (CO₂/O₂), and automated dispensing, making it ideal for long-term kinetic studies and high-throughput applications.