NextGen-O2k Fluo (Oroboros Instruments)

The Oroboros NextGen-O2k Fluo is an advanced, high-resolution respirometry and fluorescence platform designed for real-time assessment of mitochondrial function, bioenergetics, and cellular metabolism. The system enables simultaneous measurement of oxygen consumption and additional fluorometric signals under precisely controlled experimental conditions. Thanks to its highly sensitive O2k chambers and integrated fluorescence modules, the NextGen-O2k Fluo provides continuous, quantitative data on mitochondrial physiology that cannot be captured by endpoint assays.

The instrument supports automated and user-guided protocols, ensuring stable temperature control, accurate stirring, and optimal oxygen dynamics for a wide range of biological samples, including isolated mitochondria, intact cells, permeabilized cells, tissue biopsies, and small organisms. Its modular design allows flexible assay configuration and multi-parameter analysis within a single experiment.

Main applications include:

· High-resolution respirometry (oxygen consumption and flux analysis);

· Real-time monitoring of mitochondrial respiration states;

· Simultaneous measurement of fluorometric indicators (e.g., ROS production, mitochondrial membrane potential, ATP levels, Ca2+ fluxes);

· SUIT protocols for comprehensive substrate–uncoupler–inhibitor titration;

· Assessment of mitochondrial coupling efficiency and bioenergetic capacity;

· Mitochondrial function analysis in cell culture, isolated mitochondria, permeabilized fibers and tissue samples;

· Evaluation of metabolic stress, toxicity, and drug response;

· Comparative bioenergetics across species, tissues, and experimental conditions.