Molecolar Biology Lab

It deepens in studies of gene and protein expression, studies of interactions between macromolecules (DNA, proteins, RNA), studies of epigenetics and techniques of manipulation of nucleic acids.

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Activities and skills

The laboratory is equipped with cutting-edge instrumentation for the isolation and study of nucleic acids and proteins from different types of biological samples (e.g. blood, cells, tissues, saliva etc).

Researchers can use the automated nucleic acid extractor (MaxWell 16 System, Promega), quantify RNA / DNA with the Nanodrop and subsequently evaluate the expression of transcripts using QuantStudio 5 Real Time PCR System or study rare variants or perform absolute quantifications using QuantStudio 3D Digital PCR System.

Numerous activities can be carried out in the laboratory including:
-Extraction of nucleic acids using the automated MaxWell 16 System extractor (Promega)
-Gene expression study using QuantStudio 5 Real Time PCR System (Applied Biosystems)
-Absolute quantification of nucleic acids, analysis of rare variants or copy number variation using QuantStudio 3D Digital PCR System (Applied Biosystems)
-Protein extraction and Western Blotting

Instrumentation

· Automatic DNA/RNA extractor (MaxWell 16 System, Promega)
· QuantStudio 5 Real Time PCR System (Applied Biosystems) and Thermalcyclers
· QuantStudio 3D Digital PCR System (Applied Biosystems)
· Horizontal (agarose) or vertical (PAGE) gel electrophoresis chambers and transfer (Western Blotting)
· ChemiDoc XRS System (Biorad)
· Incubating Orbital Shakers for bacteria
· Nanodrop 1000 (Thermo Fisher) and Spectrophotometer Biomate 3 (Thermo Scientific)
· Benchtop and refrigerated centrifuges, Shakers, thermomixers and thermostatic baths
· Biohazard Flow Laminar Hoods

QuantStudio 3D Digital PCR System (Applied Biosystems)

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QuantStudio 5 Real Time PCR System (Applied Biosystems)

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Contact

Dr.ssa Giulia Piazzi

Via Massarenti 9, Pad 20, 4° piano 40138 - Bologna - ITALIA

+39 051 2143902 +39 051 2144903

Write an e-mail

Dr.ssa Paola Paterini

Via Massarenti 9, Pad 20, 4° piano 40138 - Bologna - ITALIA

+39 051 2143902 +39 051 2144903

Write an e-mail